Vitamine Requirements of Yeasts. 199 



with fuller's earth. By this method it is now possible to separate 

 the vitamine active for yeast growth, which has been provisionally 

 called "vitamine D," from that of the anti-beriberi or B vitamine. 



table h 



Effect on Growth of Yeast Cells and Streptococci of Shaking of 

 Autolyzed Yeast with Fuller's Earth and Norit. 



No. 





Yeast 

 Growth. 



Bacterial 

 Growth. 





Fuller's Earth: 













4- 4- 



1 r 



2. 



shaken with 50 grams per liter. . . . 



9.5 



+ + 



2(a). 



Baryta extract of fuller's earth (from 2) 



5-5 



+ + 



3- 



Autolyzed yeast (filtrate from 2) shaken with 100 











5-0 





3(a). 





4.0 



+ + 



4- 



Autolyzed yeast (filtrate from 3) shaken with 100 









grams per liter 



0.5 





4(a). 



Baryta extract of fuller's earth (from 4) 



0.0 







Norit: 







5- 



Autolyzed yeast shaken with 50 grams per liter. . . . 



10.5 



+ 



5(a). 





4.0 



+ + 



6. 



Autolyzed yeast (filtrate from 5) shaken with 100 











3-0 





6(a). 





3-0 



+ 



7. 



Autolyzed yeast (filtrate from 6) shaken with 100 











0.0 





7(a). 





O.O j 





TABLE II. 



Showing Effect on Growth of Yeast Cells and Streptococci of Fractional 

 Shaking of Beef-heart Infusions with Fuller's Earth and Norit. 







Yeast 



Bacterial 



No. 





Growth. 



Growth. 





Fuller's Earth: 







1. 



Beef-heart infusion 1 cc [equiv. to (f gm.) beef- 











12.0 



+ + 





Beef-heart infusion shaken with 50 grams per liter. 



3-0 1 



+ + 



2(a). 





1.0 



+ 



3- 



Beef-heart infusion (filtrate from 2) shaken with 100 











0.0 





3(a). 





1.0 



+ 





Norit: 







4- 



Beef-heart infusion shaken with 20 grams (2 %) per 









liter 



0.0 



+ 



4(a). 





7-5 1 



+ + 



5- 



Beef-heart infusion (filtrate from 4) shaken with 50 











0.0 





5(a). 





0.0 





Average result of several extractions. 



