222 



Scientific Proceedings (121). 



98 (1845) 



Concerning the amount and distribution of stainable lipoid mate- 

 rial in renal epithelium in normal and acutely nephropathy 

 animals, with observations on the functional re- 

 sponse of the kidney. 



By WM. DEB. MACNIDER. 



[From the Laboratory of Pharmacology of the University of North 

 Carolina, Chapel Hill, North Carolina.] 



The following observations are based upon a study of twenty- 

 six dogs. The animals w r ere under two years old. The dogs were 

 placed in metabolism cages and studied for eight days prior to any 

 experimental interference. During this period, as well as during 

 the period of the experiments, the animals were given 250 c.c. 

 of water twice a day by stomach tube. The diet consisted of 

 scraps of bread and cooked meat. The urine was collected once 

 a day and examined qualitatively for albumin and glucose. The 

 phenolsulphonephthalein test for renal function was made every 

 other day according to the technique of Rowntree and Geraghty. 

 Daily deteiminations of the alkali reserve of the blood (R.p.H.) 

 were made by the method of Marriott. Blood-urea determinations 

 were made by the method of Marshall as modified by Van Slyke 

 and Cullen. 



At the end of the eight-day period allowed for normal observa- 

 tions, seven of the animals were killed without the use of an 

 anesthetic and used as control experiments. The remaining nine- 

 teen animals were given one subcutaneous injection of 6 mgs. of 

 uranium nitrate per kilogram. Following the use of uranium, 

 observations similar to those previously outlined were continued. 

 The animals that were given uranium were killed without the use 

 of an anesthetic 6 hours, 12 hours, 24 hours and 48 hours following 

 the commencement of the intoxication. At the termination of the 

 observations on both the normal control animals and the acutely 

 nephropathic animals, kidney tissue was at once obtained for 

 microscopic study. Sections of tissue extending through each 

 lateral half of both kidneys were placed in isotonic salt solution 



