224 



Scientific Proceedings (121). 



Acutely Nephrofathic Animals. 



The experiments on the animals acutely nephropathic from 

 uranium were terminated as follows. Four animals were killed 

 six hours after the administration of uranium, five at the end of 

 twelve hours, five at the end of twenty-four hours and the remain- 

 ing five animals at the end of forty-eight hours. 



The four animals killed six hours following the administration 

 of uranium showed no change in the normal functional response 

 of the kidney. During this period the animal of Experiment 4 

 formed 129 c.c. of urine. The urine was free from both albumin 

 and glucose. Casts were not present. The elimination of phenol- 

 sulphonephthalein during the last two hours of the intoxication 

 was 75 per cent, as compared with the output of 72 per cent, of 

 the dye prior to the use of uranium. The blood urea remained 

 unchanged from the normal reading of 18 mg. per 100 c.c. of blood. 

 The reserve alkali of the blood was unaffected and remained 

 at the normal reading of 8.1. 



A study of the kidneys of the four animals killed at this early 

 period of the intoxication shows the glomeruli to be normal. 

 Stainable lipoid is not present in the endothelium of the capil- 

 laries. The stainable lipoid in the cells of the loops of Henle, 

 especially in the cells of the ascending limb of the loop, shows an 

 increase in amount when compared with the amount of such ma- 

 terial that can be demonstrated in this location in the normal con- 

 trol animals. The lipoid is in the form of granules and well- 

 defined droplets. The convoluted tubule epithelium failed to 

 show stainable lipoid with Scharlach R. at this early stage of the 

 intoxication. These cells appear normal. 



The five animals killed at the end of twelve hours following 

 the use of uranium have all shown some change from the normal 

 in the functional response of the kidney. In the urine from three 

 of the animals both albumin and glucose were present. The urine 

 of the two remaining animals contained a trace of glucose but no 

 albumin. The results obtained in Experiment 7 are characteristic 

 for this group. During the twelve-hour period of the experiment 

 the animal formed 322 c.c. of urine. Heavy traces of both albumin 

 and glucose were present. The elimination of phenolsulpho- 

 nephthalein was reduced from the normal of 70 per cent, to 55 



