Lipoid Material in Renal Epithelium. 225 



per cent. Blood urea was unchanged. The reserve alkali of the 

 blood was reduced from 8.05 to 7.95. 



Frozen sections from the kidneys of this group of animals 

 when stained with Scharlach R. show an increase in the amount of 

 stainable lipoid in the cells of the loops of Henle and, furthermore, 

 at this stage of the uranium intoxication, stainable lipoid in the 

 form of dust-like particles appears in the convoluted tubule 

 epithelium. The granules are more marked in the periphery of 

 the cells than in the area immediately around the nuclei. Other 

 than these changes in the stainable lipoid content of the tubular 

 epithelium the kidney tissue appears normal. A study of the 

 course of the intoxication to this point shows the first evidence 

 of an injury to the kidney from uranium to consist of inducing 

 such a disturbance in the cells of the loops of Henle that an increase 

 over the noimal of stainable lipoid can be demonstrated in these 

 cells. Such a change in the stainable lipoid content of these cells 

 is not associated with any functional disturbance on the part 

 of the kidney. At a later period in the intoxication, after twelve 

 hours, lipoid material stainable with Scharlach R. appears in the 

 convoluted tubule epithelium. With this evidence of injury to 

 these cells the elimination of phenolsulphonephhtalein is reduced ; 

 there is a beginning depletion in the alkali reserve of the blood, 

 and albumin and glucose or glucose alone appear in the urine. The 

 total output of urine in such animals is apparently unaffected. 



Five animals were killed at the end of the twenty-four-hour 

 period of the intoxication. The formation of urine at this stage 

 of the experiments shows an increase over the normal daily output 

 for the respective animals. The urine from all of the animals 

 shows albumin and glucose. Granular casts are present. The 

 elimination of phenolsulphonephthalein is further reduced. There 

 is no retention of blood urea. The reserve alkali of the blood was 

 depleted in all of the animals. 



Experiment 10 is representative of the group. The average 

 daily output of urine for this animal before the commencement of 

 the intoxication was 410 c.c. The urine increased to 618 c.c. on 

 the first day following the use of uranium. The urine contained 

 1.8 gm. of albumin per liter and 1.1 per cent, glucose. The 

 elimination of phenolsulphonephthalein was reduced from the 



