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Scientific Proceedings (121). 



normal of 75 per cent, to 30 per cent. There was no retention of 

 blood urea. The reserve alkali of the blood was reduced from 

 8.05 to 7.9. 



Frozen sections from the kidneys of the animals at this stage 

 of the intoxication after staining with Scharlach R. show very 

 little change in the amount of stainable lipoid in the cells of the 

 loops of Henle. Such material is abundant and in the form of 

 droplets and fused masses. There is a marked increase in the 

 amount of stainable lipoid in the cells of the convoluted tubules. 

 In this portion of the tubule the small particles that have been 

 described as appearing in this location at an earlier period of the 

 intoxication have fused so as to form small droplets which are 

 numerous. In addition to this change in the convoluted tubule 

 epithelium, these cells show marked cloudy swelling and a com- 

 mencing vacuolation. The capillaries of the glomeruli are en- 

 gorged with blood. They fail to show the presence of stainable 

 lipoid or other evidence of injury. 



The remaining five animals were killed at the end of forty- 

 eight hours of the intoxication. In two of the animals the forma- 

 tion of urine was in excess of the normal daily output. In three 

 of the animals there was a reduction in urine formation. Urine 

 from all of the animals has shown an increase in albumin and 

 glucose over that observed at the end of twenty-four hours of the 

 intoxication. The elimination of phenosulphonephthalein has 

 shown a progressive decrease. Only two of the animals show a 

 retention of blood urea. The reserve alkali of the blood shows a 

 progessive depletion. The results obtained in Experiment 16 are 

 representative of the group. The formation of urine was reduced 

 from the average normal daily output of 421 c.c. to 248 c.c. The 

 urine contained 4.7 gm. of albumin per liter and 2.08 per cent, 

 glucose. The elimination of phenolsulphonephthalein was reduced 

 from the normal output of 68 per cent, to 10 per cent. Blood urea 

 had increased from 14 to 42 mg, per 100 c.c. of blood. The reserve 

 alkali of the blood was reduced from 8.1 to 7.85. 



Frozen sections from the kidneys of these animals when stained 

 with Scharlach R. show little if any increase in the amount of 

 stainable lipoid material in the cells of the'loops of Henle or in the 

 convoluted tubule epithelium. Other changes of degeneration in 



