Antigen Removed from Circulation. 271 



serum produces little or no acceleration of the disappearance of 

 antigen it seems justifiable to assume that intravascular union 

 plays an unimportant role in the mechanism for removal of foreign 

 serum from the circulation, and therefore that the cellular phase 

 is of predominating significance. 



It is also evident from the results on the whole group of 28 

 rabbits studied that individual variation extends over a wide 

 range both in ability to form antibody and in the rate at which 

 foreign serum is removed from the circulation. 



118 (i865) 



The effect of various proteins on streptolysin production. 



By FRANKLIN A. STEVENS and CLIFFORD LAMAR. 



[From the Department of Medicine, College of Physicians and Sur- 

 geons, Columbia University, and the Presbyterian Hospital, 

 New York City.] 



Variations in streptolysin production in horse and rabbit 

 serum media were noted in a previous publication. In horse 

 serum hemolysin was produced in titratable quantities later in 

 the growth of cultures than in rabbit serum broth but the maximum 

 concentration reached was greater. Unless glucose were present 

 the curve of lysin production corresponded closely to that of 

 growth since hemolysin was found in greater concentration during 

 the period in which the bacteria were multiplying most rapidly. 

 On account of these differences which were characteristic of these 

 sera an attempt was made to discover the responsible factors. 

 The albumen and globulin ratio was modified so that the horse 

 serum contained the same proportions of horse-serum albumen 

 and globulin as were found in rabbit serum, and rabbit serum the 

 same percentages as were present in horse serum. Flasks pre- 

 pared with 20 per cent, of these modified sera in plain infusion 

 with 0.7 per cent. NaCl, were seeded with equal quantities of a 

 16-hour culture of hemolytic streptococcus in 20 per cent, horse 

 serum broth. Hemolysin titrations were then made at intervals 

 of an hour with a suspension of horse corpuscles in physiological 

 salt. 



The percentages of albumen and globulin in normal rabbit 



