Streptolysin Production. 



273 



When streptococci which had not been animal-passed were 

 grown in flasks of these media so that comparisons of growth and 

 hemolysin could be made, the growth was more luxuriant, the 

 lag shorter and the hemolysin stronger in the peptone which had 

 been only partially hydrolyzed. This suggests that causes for 

 variations in growth are to be sought in the partially split proteins 

 of the blood serum. 



119 (1866) 



The bicarbonate and chloride content of the blood in certain 

 cases of persistent vomiting. 



By H. A. MURRAY, JR. (by invitation). 



[From the Presbyterian Hospital, New York City.] 



These researches were instigated by an interest in the phenom- 

 enon of tetany, particularly in that form known as gastric tetany. 

 Very little work has been done in this field on human subjects, 

 none recently; and it seems that no blood analyses have been 

 published. Whatever work was done was not convincing and 

 the old hypotheses such as the dehydration and mechanical 

 theories once offered as a result of clinical studies to explain the 

 condition must be discarded as untenable. 



There has been some successful experimental work on dogs, 

 however, in which tetany was produced by obstructing the pylorus 

 and in which various disturbances in the salts of the blood were 

 recorded. 



To summarize: McCann 1 (191 8) was the one to discover that 

 after pyloric closure there was a rise in the combined carbon di- 

 oxide. This was confirmed by MacCallum 2 (1920) and in the 

 surgical laboratories of this college (Hastings 3 and Murray 192 1). 

 MacCallum and ourselves also found a markedly diminished 

 chloride content with normal values for calcium. In our lab- 

 oratory, contrary to expectations, it was found that the H-ion 



1 McCann, W. S. J. Biol. Chem., 1918, xxxv, 553. 



2 MacCallum, W. G„ Lintz, J., Vermilye, H. N„ Leggett, T. H., and Boas, E. 

 Bull. Johns Hopkins Hosp., 1920, xxxi, 1. 



3 Hastings, A. B., Murray, C. D., and Murray, H. A. J.Biol. Chem., 1921, 

 xlvi, 223. 



