Studies in the Physiology of Vitamins. 



283 



extract administered either with single large doses or with large 

 doses repeated daily for as many as fourteen (14) days, the material 

 being introduced by stomach sound in order to eliminate the taste 

 factor. In another series of experiments the meat extract was 

 mixed with the food which had been refused. The meat extract 

 did not restore the appetite to such animals. On the other hand 

 relatively small amounts of the yeast extract given by stomach 

 produced a prompt recovery of appetite which lasted for from 

 four (4) to nineteen (19) days depending on the amount ad- 

 ministered. 



Liebig's extract in doses such as were employed in these ex- 

 periments promotes the flow of gastric juice in normal animals. 2 

 This fact, together with our own observation that products con- 

 taining vitamin-B do not promote the flow of saliva, pancreatic 

 juice, or bile, 3 suggests that the recovery of the desire to eat in our 

 animals is not to be ascribed to an increased flow of gastric juice. 



124 (1871) 



Studies in the physiology of vitamins. IV. Parenteral administra- 

 tion of products containing vitamin-B — 

 mammalian experiments. 



By GEORGE R. COWGILL. 



[From the Sheffield Laboratory of Physiological Chemistry in Yale 

 University, New Haven, Conn.] 



That relief from the symptoms due to a lack of vitamin-B in 

 pigeons may be brought about in a very short time by intra- 

 muscular injection of products containing this vitamin has been 

 shown by many investigators. Studies of parenteral administra- 

 tions of vitamin-B to mammals do not appear to have been made. 

 In the course of our studies into the physiology of this vitamin, 

 using dogs as experimental animals, the protein-free concentrate 

 of vitamin-B from yeast as prepared by the Harris Laboratories 



2 Pawlow, "The Digestive Glands," 1902, 96. 



3 Cowgill, Proceedings Soc. Exp. Biol, and Med., 1921, xviii, 148-149; 

 ibid., 290. 



