302 



Society Proceedings (122). 



4. A modified "N.N.N." medium is suggested. 



5. On this medium the Leishman-Donovan bodies from spleen 

 punctures or peripheral blood develop into flagellates at all 

 hydrogen-ion concentrations tested, i.e., between P H 6.8 and 

 P H 8.2 and the flagellates grow at least to P H 9.0. 



6. Cultures on this modified medium show post-flagellate 

 forms and perhaps Cornwall's "thick tails." 



Abstracts of the Communications, 

 Pacific Coast Branch. 



San Francisco, California, February 15, IQ22. 

 136 (1883) 



Further observations on anaphylactoid phenomena from different 

 agents, including histamin. 1 



By PAUL J. HANZLIK and HOWARD T. KARSNER. 



[From the Department of Pharmacology, Leland Stanford Junior 

 University, San Francisco, Cat., and the Department 

 of Pathology, Western Reserve University, 

 Cleveland, Ohio.] 



Of the twenty-five different agents which were injected intra- 

 venously into guinea pigs, the following caused anaphylactoid 

 symptoms: Colloidal arsenic, kaolin, blood charcoal, colloidal 

 iron, ten per cent, sodium chloride (?), tragacanth, toxified agar, 

 lung extract, glacial acetic acid, copper sulphate, fuller's earth, 

 sodium oxalate, sodium citrate, tannin, tartar emetic and histamin. 

 Especially noteworthy were the results after injection of histamin, 

 which produced symptoms with the very small dosage of 0.0001 1 

 mgm. per gram of animal. All of these agents, except the chloride 

 and citrate, produced thrombi in the pulmonary blood vessels. 

 The appearance of pulmonary thrombi (platelet) after the injec- 

 tion of histamin agrees with the observation of Dale and Laidlaw, 

 who detected the presence of platelet thrombi in the blood of 



1 This investigation is supported by a grant from the Therapeutic Research 

 Committee of the Council of Pharmacy and Chemistry of the American Medical 

 Association. 



