324 



Scientific Proceedings (123). 



than that obtained with the uric acid alone. The reaction was 

 not noted in normal controls. At that time we stated that we 

 did not know whether phenol was the cause of this reaction. 



That the so-called phenol reagent (phosphotungstate-phospho- 

 molybdic reagent) first described by Folin and Denis 1 was not 

 specific was shown by Tisdale, 2 Gortner and Holmes 3 and others. 

 They showed that there were other substances which might be 

 present in the blood that gave the same color reaction, hence in- 

 terfering with the purpose of the original test. A deep blue color 

 is also given by lactic acid, by indol, indol derivatives, protein 

 derivatives and many other substances. 



In order to eliminate at least one group of these substances, 

 we determined the amino-acid nitrogen and the peptid nitrogen 

 in some of these cases, using the method described by Van Slyke 

 and Whipple. 4 Although the urea was high in some of these 

 cases the amino-nitrogen and the peptid-nitrogen values were 

 normal, or slightly above normal. One must remember, how- 

 ever, that there may be some toxic protein derivative products 

 or amino acids present which are very toxic in small amounts, 

 although not sufficient to perceptibly increase the amino or pep- 

 tid nitrogen in the blood. 







Mgs. per 100 c.c. 





Hospital No. 











Remarks. 





Amino N. 





Peptid N. 



Urea N. 





212138 



12 





14 



19.6 



Recovered 





12 





14 



IS- 



Died 



212352 



14 





18 



40. 



Died 



212338 



14 





17 



19.6 



Died 





15-4 





17.1 



14.2 



Recovered 



151 (1898) 



The influence of sodium citrate on peristalsis. 



By WILLIAM SALANT and NATHANIEL KLEITMAN. 



[From the Department oj Physiology and Pharmacology , University of 

 Georgia, Augusta, Georgia.] 

 The Trendelenburg method was used for the study of intestinal 

 movements in anesthetized animals. Doses of 30-60 mgs. sodium 



1 Folin, O. and Denis, W., J. Biol. Chem., 1912, xii, 239. 



2 Tisdale. F. F., J. Biol. Chem., 1920, xliv, 409. 



' Gortner, R. A., and Holmes, G. E., J. Am. Chem. Soc, 1920, lxii, 1678. 

 4 Whipple, G. H., and Van Slyke. D. D., /. Exp. Med., 1918. xxviii. 213. 



