Antigenic Properties of Red Cell Globulin. 345 



used. These results indicate that the reactions attending the use 

 of citrate must be sought by other criteria. They may be asso- 

 ciated with the disturbance of equilibrium causing the precipitate 

 above mentioned. 



ABSTRACT OF THE COMMUNICATIONS, PACIFIC COAST BRANCH. 



Thirty-third meeting. 



Berkeley, California, April 12, IQ22. 

 166 (1913) 



The antigenic properties of red-cell globulin. 1 

 By CARL L. A. SCHMIDT and D. E. DEMENT. 



[From the Department of Biochemistry and Pharmacology, University 

 of California, Berkeley, Cal.] 



Three theories have recently been advanced relative to the 

 nature of the antigen which, on repeated injection of foreign red 

 cells, gives rise to a specific hemolytic sensitizer in the blood stream 

 of the immunized animal. Balls and Korns 2 have come to the 

 conclusion that the antigen is contained in the stroma of the red 

 cell and that it is neither a globulin nor an albumin but probably 

 a nucleoprotein. While the presence of nucleic-acid residues in 

 non-nucleated red cells cannot be denied, the careful work of 

 Bloor 3 indicates that within the limits of experimental error of his 

 method the presence of nucleoprotein in red cells appears doubt- 

 ful. The experiments of Wooldridge 4 on the constituents of the 

 stroma of red cells shows that although a protein combined with a 

 molecule containing phosphorus (this may be lecithin) is present 

 in small quantities, the greater part of the protein fraction con- 

 sists of paraglobulin. 



While it is not to be denied that immunization with stroma 

 does lead to the appearance of a hemolytic sensitizer, the experi- 



1 Aided in part by a grant from the Research Board of the University. 



2 Balls, A. K., and Korns, J. R., Jour, of Immunology, 1918, iii, 375. 



3 Bloor, W. R., Jour. Biol. Chem., 191 8, xxxvi, 49. 



4 Wooldridge, L., Arch. f. Anal. u. Physiol. (Physiol. AM.). 1881, p. 387. 



