348 



Scientific Proceedings (123). 



titer of the last three sera. However it is frequently found that 

 only comparatively low titer sera are obtained when rabbits are 

 immunized with human red cells. 

 These experiments definitely indicate that immunization with 

 the C0 2 -globulin prepared from the water-soluble portion of the 

 red cells of the sheep, the pig and man leads to the appearance, in 

 the blood stream of the treated animal, of a specific hemolytic 

 sensitizer and of an agglutinin. It appears possible, although our 

 experiments are incomplete on this subject, that the globulin from 

 the water-soluble portion of the red cell is closely related to one of 

 the proteins contained in the stroma, since by immunization with 

 either of these antigens, the same antibody is obtained. 



167 (1914) 



The separation of the hexone bases from a protein hydrolysate by 



electrolysis. 1 



By G. L. FOSTER and CARL L. A. SCHMIDT. 



[From the Department of Biochemistry and Pharmacology of the 

 University of California , Berkley, Cal.] 



In a previous communication describing a method for the 

 preparation of glutamic acid, the need of developing cheaper 

 methods for the production of amino acids in quantity was pointed 

 out. This is especially true with respect to the amino acids 

 arginin, histidin and lysin. The cost of reagents and the labor 

 required for the preparation of these amino acids prohibits experi- 

 mental work in which large quantities of these substances are 

 required. 



Some years ago Ikeda and Suzuki 2 described a method for 

 separating certain fractions of the products of protein hydrolysis. 

 Their method has apparently not come into general use and ex- 

 perimental data are not available. On passing direct current 

 through a solution of the protein cleavage products, which is 

 placed in the center of a three-compartment cell, the amino acids 

 are separated into three fractions consisting of (a) the amino 



1 Aided by a grant from the Research Board of the University. 



1 Ikeda, K., and Suzuki, S., U. S. Patent No. 1015891, Jan. 30, 1912. 



