Immunologic Studies of Actinomycetes. 



35i 



Distribution of Nitrogen in* the Gelatin Hydrolysate 

 Before and After Electrolysis. 





Nitrogen in orig- 

 inal Hydroly- 

 sate. 1 



Distribution 

 of Basic Nitro- 

 gen in Gelatin 

 as Found by 

 Van Slyke,* 

 %. 



Cathode Solu- 

 tion after Elec- 

 trolysis at 

 P H 7-5.- 



Cathode Solu- 

 tion after Elec- 

 trolysis at 

 P H S-5« 



Nitro- 

 gen, 

 Gms. 



Distri- 

 bution 

 of Basic 

 Nitro- 

 gen, 

 %• 



Nitro- 

 gen, 

 Gms. 



Distri- 

 bution 

 of basic 

 Nitro- 

 gen, 

 %. 



Nitro- 

 gen, 

 Gms. 



Distri- 

 bution 

 of Basic 

 Nitro- 

 gen, 

 %• 





61.0 







6.21 





O.99 





Total basic nitrogen .... 



18.5 







6.19 





0.99 







10. 1 



55 



58 



4.70 



76 



O.62 



63 



Histidin nitrogen 



3-6 



19 



17 



0 



0 



O.15 



15 





4.8 



26 



25 



I.49 



24 



0.22 



22 



Abstracts of the Communications, Minnesota Branch. 

 Fourth meeting. 

 Minneapolis, Minnesota, April 12, 1922. 



168 (1915) 



Immunologic studies of actinomycetes, with special reference to 

 the acid-fast species. 



By EDMOND NELSON and ARTHUR T. HENRICI. 



[From the Department of Bacteriology and Immunology, the Uni- 

 versity of Minnesota, Minneapolis, Minn.] 



The serums of six out of eight actinomycotic cattle fixed com- 

 plement with four different antigens prepared from Actinomycetes 

 isolated aerobically from lesions in cattle, and not with antigens 

 prepared from saprophytic strains. The complement fixation test 

 may be of diagnostic value in this disease. 



The serum of a rabbit immunized against A. bovis fixed com- 

 plement with antigens prepared from A . bovis and A . madurce, but 

 not with antigens prepared from the acid-fast varieties A. aster- 

 oides and A. gypsoides, or from Mycobacterium tuberculosis. The 



1 500 gms. of gelatin were hydrolyzed and the volume was brought to 2,000 c.c. 



2 Van Slyke, D.D., J. Biol. Chem., 191 1, x, 49. 



3 1,000 c.c. of the original hydrolysate were used for this experiment. 



4 130 c.c. of the original hydrolysate were used in this experiment. 



