352 



Scientific Proceedings (123). 



serums of rabbits immunized against A. gypsoides fixed comple- 

 ment equally with antigens prepared from A. gypsoides and A. 

 asteroides, and in lower dilutions with M. tuberculosis, but not at 

 all with A. boots and A. madurce. The serums of rabbits immun- 

 ized against M. tuberculosis fixed complement with the homolo- 

 gous antigen, and in lower dilutions, with antigens prepared from 

 the acid-fast Actinomycetes, A. aster oides and A. gypsoides, but not 

 with A. bovis and A. madurce. It would seem, therefore, that the 

 acid-fast Actinomycetes are more closely related to the acid-fast 

 bacteria than to the non acid-fast Actinomycetes. 



A. gypsoides does not secrete a soluble toxine, but forms a very 

 protent endotoxine. By careful vaccination there can be pro- 

 duced in rabbits an active protective immunity. The serum of 

 such rabbits injected into guinea pigs gives the latter partial 

 protection. 



169 (1916) 



The effect of sodium benzoate and sodium hippurate, and other 

 drugs upon the glomerular circulation in the frog. 



By RAYMOND N. BIETER and ARTHUR D. HIRSCHFELDER. 



[From the Department of Pharmacology, University of Minnesota 

 Minneapolis, Minnesota.] 



The effect of injection of sodium benzoate and sodium hippu- 

 rate on the circulation through the glomeruli of frogs' kidneys 

 was studied by Richards's 1 method of direct observation. Sodium 

 benzoate, .008 to .020 mil of a ten per cent, solution per gram 

 frog, invariably causes increased circulation through the glomeruli, 

 increasing both the number of functionating glomeruli, the 

 number of active loops in each glomerulus, and the velocity of 

 flow in each capillary. Sodium hippurate, .008 to .020 mil of a 

 fourteen per cent, solution per gram frog, had the opposite effect. 

 This harmonizes with the work of H. B. Lewis, 2 and F. B. Kings- 

 bury and W. W. Swanson, 3 who demonstrated that sodium hip- 



1 Richards, A. N., Am. J. Med. Sc., 1922, clxiii, No. 1. 



1 Lewis, H. B., /. Biol. Chem., 1914, xviii, 225. Lewis and Karr, /. Biol. Chem. 

 1916, xxv. 13. 



* Kingsbury, F. B., and Swanson, W. W., Arch. Int. Med., 1921, xxviii, 220-36. 



