358 



Scientific Proceedings (123). 



Four rats of litter 23, twenty days old, weighing 27 d= 1 gm. were 

 taken and placed on these diets. From the 33d to the 40th day 

 of age they were kept in metabolism cages and the calcium phos- 

 phate metabolism determined with the following results: 









Grams. 



Milligrams per Day. 





Rat 

 No. 



Sex. 



Diet. 



Body Weight. 



Food 

 Intake 



P. 



Ca. 



X-Ray 46 

 Days Old. 









33 

 Davs. 

 Old. 



40 

 Davs 

 Old. 



Gain 



per 

 Day. 



In. 



Out. 



Ret. 



In. 



Out. 



Ret. 





1 



0 



96 



33 



34 



I 



3-49 



4.2 



3-1 



I.I 



21.2 



19.7 



1-5 



Rickets 



11 



9 



98 



36 



42 



6 



3.8, 



5-1 



3-5 



1.6 



237 



22.7 



1.0 



Rickets 



III 



9 



99 



47 



65 



18 



7.50 



21.4 



13.8 



7-6 



48.4 



38.1 



10.3 



No 



Rickets 



IV 



0 



100 



52 



73 



21 



7-36 



25.O 



15-8 



9.2 



46.7 



32.7 



14.0 



No 



Rickets 



It will be seen from the table that diets 99 and 100 containing 

 the second clear and the Graham flours caused retention of P 

 and Ca and prevented rickets, other things being equal. The X- 

 ray plate suggested a very mild rickets in the rat eating Graham 

 flour. Diet 96, containing high alkali, stopped the growth as 

 well as produced rickets. We may conclude that it would be 

 safer to feed infants Graham bread instead of white bread, but if 

 the Graham bread is too laxative, bread may be made of second 

 clear flour, which is not laxative, and rickets be prevented with 

 more certainty than with the Graham flour. 



Methods. — The rats were placed in wire cages sitting in six- 

 inch silica dishes and at the end of the metabolism period the cages 

 were lifted out, the dishes placed in a muffle and ashed at the low- 

 est possible temperature in an atmosphere of O2. The ash was 

 then dissolved in dilute nitric acid and evaporated and redissolved 

 and boiled. 



Ca Analysis. — Take aliquot containing 10-50 mg. Ca in a 200 

 c.c. pyrex flask, add I drop brom-phenol blue, and 20 c.c. 2 }4 per 

 cent, oxalic acid; add 20 per cent. Na 2 C0 3 drop by drop until color 

 changes to lavender; boil; stopper and shake one hour; filter; wash; 

 transfer precipitate back to 200 c.c. flask with 100 c.c. H 2 0; add 5 

 c.c. cone. H 2 S0 4 ; heat to 75 0 and titrate drop by drop with 0.1 N 



