Glomerular Circulation in Frog. 



4i5 



infected material as the stomach contents of an aborted fetus. 

 After two or three transfers, the bacilli grow quite readily even 

 under ordinary aerobic conditions. 



We have been using the method described by Huddleson, but 

 have used in place of a CO2 generator described by him, commer- 

 cial CO2 from a tank. This is because too much chlorine was 

 given off with the gas from the Huddleson generator. We also 

 used a serum agar media, the agar being made from lean beef and 

 when used 10 per cent, of naturally sterile horse serum was added to 

 the melted agar, cooled to 50 0 C, and the tubes are allowed to 

 solidify in a slanting position. The reaction of the agar is adjusted 

 to a P H of 6.8 to 7.2, which is slightly more alkaline than hereto- 

 fore recommended. . 



These culture tubes are heavily seeded with the material to 

 be cultured, and are placed in a Whitall Tatum museum jar to 

 which 10 per cent. C0 2 is added. The jar is then sealed and placed 

 in an incubator at 37^° C. After 24 hours' incubation, small 

 pin-point colonies will be observed, and after 48 hours' incuba- 

 tion, well-developed colonies of Bad. abortus will be noticed. 



Huddleson makes the statement that C0 2 accelerates and 

 favors the growth of Bad. abortus. We have been able to obtain 

 the same results, using 10 per cent, hydrogen. It would there- 

 fore seem that the diminished oxygen tension rather than any 

 specific effect of the CO2, is involved. This last was suggested by 

 Edwards in his review of the article by Huddleson. 



205 (1952) 



The effect of phenolsulphonephthalein upon the glomerular 

 circulation in the frog. 



By ARTHUR D. HIRSCHFELDER and RAYMOND BIETER. 



[From the Department of Pharmacology, University of Minnesota, 

 Minneapolis, Minn.] 



Further studies upon the function of the frog's glomeruli ob- 

 served by the method of A. N. Richards 1 have shown that phenol- 

 sulphonephthalein is also excreted through the glomeruli. After 



1 Richards, A. N., Am. J. Med. Sc., 1922, clxiii, 1. 



