420 



Scientific Proceedings (124). 



two parts in each case that it may be considered the same despite 

 the great difference in structure. 



table 1. 



Shrinkage Produced by the Technique when the Posterior and Anterior 

 Lobes were Separated from Each Other. 



Autopsy Number. 



20-446. 



20-450. 



22-140. 



Lobe 



Anterior. 



Posterior. 



Anterior. 



Posterior. 



Anterior. 



Posterior. 



Original volume, 



Final volume, c.c. . 

 Shrinkage, c.c. . . . 

 Per cent, of shrink- 

 age 



•3632 

 .2430 

 .1202 



33-1 



.1415 

 .0950 

 .0465 



32.9 



.3113 

 .1928 

 .1185 



38.1 



•1057 

 .0666 

 .0391 



37-0 



•2547 

 .1540 

 .1007 



39-5 



.1113 

 .0691 

 .0422 



38.0 



This is a method which has been used extensively for similar 

 purposes in this and other laboratories and especially by God- 

 lewski, Hammar and Jackson, and the senior author has used it 

 on the hypophysis of the woodchuck. Whether any time would 

 be saved by measuring the areas with a planimeter has not yet 

 been determined on this material. Dr. C. M. Jackson, of this 

 laboratory, after comparing the two methods, favored the cutting- 

 out process. The planimeter cannot increase the accuracy pro- 

 viding one has dexterity with the scissors. 



For a differential count of the types of cells in pars anterior, 

 a 5-/z section from each of the three planes mentioned is stained 

 with Mallory's connective tissue stain, going rapidly through the 

 alcohols following the stain. No better differential stain could 

 be wished than this will give on material only a few hours post 

 mortem. These three sections are systematically explored with 

 an oil-immersion lens by means of a mechanical stage, and the 

 cells of each type in each fifth field of each fifth row (as shown in 

 Fig. 1) are counted and the percentage of the total calculated. 



Results. 



In Table II are recorded some selected cases to illustrate what 

 the method yields in four normal males with hypophyses covering 

 the usual range in weight met with, one female with a rather 

 large hypophysis and one case of non-neoplastic post-adolescent 

 hypopituitarism of both lobes. The small normal male hypo- 



