22 



Scientific Proceedings (30). 



It was found that the addition of 30 per cent, of normal serum 

 was sufficient to inhibit the action of the hemolysins in both the 

 syphilitic and carcinomatous sera. 



Normal serum seems to possess protective substances which 

 inhibit the action of the hemolysins. Hemolytic sera also may 

 possibly contain the same protective substances as normal sera. 

 These substances, if present, would neutralize a certain amount of 

 the hemolysin existing in the serum. 



If only a small amount of hemolysin be present, it would, if 

 the above conditions actually exist, be neutralized and rendered 

 incapable of detection by the technique used at present. The 

 corpuscles belonging to such a serum, however, would still be im- 

 mune to hemolytic carcinomatous and syphilitic serum. 



The serum of one case of tertiary lues was found to contain so 

 little hemolysin as to be barely demonstrable. The corpuscles of 

 this blood, however, were perfectly immune to either carcinoma- 

 tous or syphilitic sera. 



The results of this research have some bearing on the subject 

 of transfusion. Inasmuch as some cases of carcinoma possess 

 hemolytic sera, we could not transfuse such cases with normal blood- 

 If transfusion of such a carcinoma case be found necessary — as 

 a preliminary to operation or for other reasons — it will be neces- 

 sary to secure as donor a person whose blood corpuscles are im- 

 mune to the serum of the carcinoma case and vice versa. 



According to our observations, the desired blood could be found 

 in cases of tertiary lues that have just recovered from their 

 lesions. 



The hemolysins found in syphilis and carcinoma appear to be 

 true isolysins. In experimental isolysin-formation the isolysin is 

 a reaction product of the organism in which it is formed. The 

 corpuscles of the animal in whom the isolysin is produced are im- 

 mune to that isolysin. No anti-hemolysins are present in the sera 

 of such animals. 



The evident immunity of the corpuscles in the hemolytic syphi- 

 litic and carcinomatous bloods towards the hemolysins in their sera 

 indicates that the hemolysin is a true isolysin, and, like the experi- 

 mental isolysins, is probably a reaction product of the organism in 

 which it occurs. Some toxic substances absorbed from the dis- 



