Canine Infection with Leishmania Infantum. 27 



The dog was bled and when autopsied on October 9 presented 

 evidence of a prolonged chronic infection. The spleen was small 

 and tough and weighed but 33 grams, the dog weighing 11.7 

 kilos. The liver and kidney likewise were found to be unusually 

 hard. Microscopical examination of the spleen, liver, kidneys, 

 lungs, and bone-marrow showed enormous numbers of typical 

 Leishman-Donovan bodies, free and intracellular, with no sign of 

 the flagellate form. Cultures made from the spleen and liver gave 

 at the end of five days exceedingly rich growths of the flagellated 

 organism. 



Tubes inoculated with the peripheral blood likewise gave good 

 cultures though somewhat later, that is, on the tenth day. The 

 latter fact indicates the value of the cultural method as a diagnostic 

 means. 



It will be seen therefore that, starting out with the flagellate 

 form, it has been possible to produce a typical infection in the dog 

 and to recover from the infected animal, by cultural means, the 

 parasite in the flagellated stage. Undoubtedly this result can also 

 be obtained by employing less massive doses than was deemed 

 necessary in this preliminary experiment. 



14 (352) 



New apparatus designed especially to facilitate the preserva- 

 tion of food for use in metabolism experiments.^ 



A demonstration. 



By WILLIAM J. GlES. 



{From ike Laboratory of Biological Chemistry of Columbia Utii- 

 versity, at the College of Physicians and Surgeons^ 



The writer exhibited a new form of apparatus that has been 

 very serviceable in the preservation of fresh food by refrigeration. 

 The apparatus consists in the main of a galvanized "angle iron" 

 frame constructed to support glass trays specially designed as food 

 containers. Fresh food, e. g., hashed meat, may be very satis- 

 factorily preserved, without change of general composition, by 



' This method further improves the process described by the author some years ago 

 in the American Journal of Physiology (190I, v, p. 235). See also Gies and col- 

 laborators : Biochemical Researches, 1 903, i, p. 69 (Reprint No. i). 



