Lysins, Precipitins, Agglutinins., Opsonins. 59 



during anesthesia or inhibition. This view is supported by a con- 

 sideration of the electrical changes accompanying inhibition and 

 stimulation, respectively ("positive" and " negative variations"). 



26 (364) 



On the relative concentration of lysins, precipitins, agglutinins, 

 opsonins and related substances in the different body 

 fluids of normal and immune animals. 



By F. C. BECHT and J. R. GREER. (By invitation.) 



\_From the Hull Physiological Laboratory of the University of 



Chicago?^ 



The present work was suggested by some of our previous 

 work with Prof. Carlson on the physiology of lymph. It was 

 suggested that it would be of profit to establish the differences 

 between scrum and the other body fluids in their content of anti- 

 bodies of various kinds in normal and immune animals, with the 

 hope that it would have some bearing upon the problem of lymph 

 formation, and also upon the origin of these antibodies. Thus far 

 the hemolysins, hemagglutinins, bacterial agglutinins, bacterial 

 opsonins, hemopsonins, and precipitins have been studied in the 

 serum, neck lymph, thoracic lymph, pericardial fluid, cerebrospinal 

 fluid, and aqueous humor. The bacterio-lysins are also under 

 consideration. 



The results with normal dogs have been the following : Hemo- 

 lysins are found in the serum, thoracic lymph, and neck lymph in 

 the normal animal. Serum and thoracic lymph contain them in 

 almost equal quantities, with a slight balance in favor of the serum. 

 The hemolytic power of the neck lymph is much lower than that 

 of the serum, and is almost entirely wanting in the lymph which 

 is secured without massage. In two of seven cases there was a 

 small amount of hemolysis in the pericardial fluid, in the remain- 

 ing cases there was no laking when the fluid was free from ery- 

 throcytes. There is no hemolysis in the cerebrospinal fluid, ex- 

 cept in one case where there was a trace of free hemoglobin. There 

 was no hemolysis in the aqueous humor. The hemagglutinins 

 run parallel with the hemolysins except that they act in higher 

 dilutions than the latter. 



