6o 



Scientific Proceedings (31). 



Bacterial agglutinins are found in the serum, thoracic lymph^ 

 and neck lymph. The two lymphs were of equal strength, and 

 the serum was approximately ten times as strong. Pericardial 

 fluid, cerebro-spinal and aqueous humor were entirely lacking in 

 these anti-bodies. No precipitins for rabbit serum are found in 

 any of the body fluids of the normal dog. 



The hemolytic power of the serum and thoracic lymph of dogs 

 immune to typhoid is slightly higher than that of the same fluids 

 of the normal animal. No increase has been noted in the other 

 fluids examined. The hemagglutinating power is increased in the 

 serum, neck lymph, thoracic lymph, and pericardial fluid, particu- 

 larly in the latter. The bacterial agglutinating power of the serum, 

 neck lymph, and thoracic lymph is much increased. The pericardial 

 fluid and aqueous humor are inactive or at most show only a trace. 

 The cerebro-spinal fluid has not yet been tested. 



In dogs rendered immune to rabbit blood we have not yet 

 noted any very marked increase in hemolysins over those of the 

 normal animal. There was a marked increase of the hemaggluti- 

 nins in the serum, neck lymph and thoracic lymph, and in one 

 case in the pericardial fluid and aqueous humor. Immunization to 

 rabbit blood has no effect on the bacterial agglutinins. The injec- 

 tion of defibrinated rabbit blood did not produce precipitins for 

 rabbit serum in any of the body fluids of the animal. The work 

 on bacterial opsonins and hemopsonins in both normal and immune 

 animals has been entirely unsatisfactory. 



The work is being continued and extended to cats and rabbits. 

 Later, lymph will be collected from various organs and extracts 

 from various tissues made with the hope of finding the source of 

 these antibodies. Parallel experiments are being made on the 

 number and kind of leucocytes in the various body fluids. 



