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Scientific Proceedings (34). 



became quite uneasy after receiving the dose. At the end of an 

 hour he showed great muscular weakness and tremor. 



Animal No. 2. — The weight of the second animal was 4.25 

 kilos. It was given a mixture of 100 c.c. of 0.2 per cent, citric 

 acid, 50 c.c. of water and 0.85 c.c, of concentrated hydrochloric 

 acid (sp. gr. 1.19). This animal was entirely unaffected. 



The same experiments were repeated on the same animals the 

 next day ; the results were practically identical. The animal (i) 

 that received the free acid-benzoic acid mixture, however, was 

 more prostrated than on the previous day and showed general stiff- 

 ness of the muscles. At the end of six hours it was chloroformed 

 and autopsied, when it was found that the stomach contained 

 " coffee ground " material. There were ecchymotic areas and some 

 places appeared to be ulcerated slightly. The intestines showed 

 marked congestion here and there and appeared to be slightly 

 ulcerated in places throughout. The grumous material in the 

 stomach and intestines gave a strong guaiac test and was undoubt- 

 edly modified blood. The liver and lungs showed considerable 

 congestion with some evidences of infarcts. The kidneys were 

 cyanotic, the cortex very much congested, while the medulla was 

 pale and anemic. 



Further investigation is contemplated, especially on the influ- 

 ence of nephrectomy on the toxicity of benzoic acid. 



I am indebted to many members of the Purdue University 

 Alumni Association of New York City for volunteering as subjects 

 in this investigation and thus making it possible for me to carry 

 out experiments on a large number of individuals. The Secretary, 

 Mr. Leslie Hustable, Mr. Ray C. Ewry, Mr. R. W. Parks, Mr. F. 

 M. Waltz and Mr. H. Worsham of that organization have given 

 me special assistance in various ways. I am also indebted to Drs. 

 A. E. Olpp and Matthew Steel, and Messrs. Hcrzfeld and Bisch 

 for cooperation, and to Drs. Foster, Mosenthal and Rosenbloom 

 for criticism and suggestions. Professor Gies has given me all the 

 facilities of his laboratory for the conduct of this research, as well 

 as valuable criticism and suggestions. 



