1 8 



Scientific Proceedings (46). 



belong to a new species, I propose for them the name Trypanosoma 



rhesii. 



2 (621) 



On the question of immunization against transplantable cancer 

 by injection of an animal's own tissues. 



By R. A. LAMBERT. 



[From the Department of Pathology, College of Physicians and 

 Surgeons, Columbia University.} 

 That the injection of a suitable quantity of homologous tissue 

 induces in susceptible mice a certain degree of resistance to the 

 inoculation of their transplantable cancers is a well-established 

 fact. There appeared recently a paper by Woglom 1 in which 

 experiments were described showing that autogenous tissue 

 (spleen) injected subcutaneously was also capable of inducing this 

 immunity. Woglom's work has been questioned by Apolant 2 who 

 repeated the experiments with negative results. Apolant main- 

 tains further that the spleen of a mouse does not afford sufficient 

 tissue for immunization, and that the question as to the possibility 

 of immunizing with an animal's own tissues is still an open one. 



In the experiments herewith reported, blood has been used as 

 the immunizing agent. The quantity of defibrinated blood neces- 

 sary for immunizing young mice was shown by Bashford to be 

 about .3 c.c. In order to eliminate, however, the possibility of 

 insufficient dosage, it was considered desirable to use at least .5 

 c.c. As Woglom stated in his paper, mice cannot be bled 

 this amount plus the loss attending the bleeding without caus- 

 ing death. Interval bleedings from the jugulars were there- 

 fore resorted to. In nearly all of the experiments two bleedings 

 only, on successive days, were necessary. About ten drops of 

 blood in citrate solution were taken each time. The corpuscles 

 were preserved in the ice box and injected subcutaneously on the 

 day after the last bleeding. Ten mice were treated in this way; 

 ten controls were injected with a similar quantity of homologous 

 blood; ten normal mice were set aside as controls on the two 



1 Jour. Exp. Med., January, 1910, p. 29. 



2 Zeil.f. Immunitdtsforschung, July, 191 1. 



