Protein Metabolism in Phlorhizin Diabetes. 55 



of sugar and nitrogen in the urine of phlorhizinized dogs. The 

 degree of reduction in the nitrogen elimination in most of their 

 dogs is so marked (less than 1 gm. per 24 hours for dogs weighing 

 5.4 to 10.0 kilos!) that a repetition of this experiment seemed 

 desirable. 



Thanks to the kindness of Prof. Graham Lusk, I received 

 100 gm. of glutaric acid, which was prepared by Kahlbaum, and 

 which enabled me to carry out the following research : 



Dogs were phlorhizinized in the usual manner, and after estab- 

 lishing the D : N ratio, they received, subcutaneously, 10 gm. of 

 glutaric acid dissolved in water and neutralized by means of 

 NaHC0 3 . The glutaric acid was administered in three equal 

 doses during the course of the day. 



Dog No. 5. 



Weight. 



Total N. 



Total Sugar. 



D:N. 



Remarks. 



12.7 



18.02 



60.96 



3.38 





12.2 



21.07 



70.32 



3-33 



10 gm. glutaric acid. 



EX.9 



19-75 



66.24 



3-35 









Dog No. 7. 







13.87 



16.92 



63-05 



3-72 







17.86 



65-54 



3-67 



10 gm. glutaric acid. 



These results show that the glutaric acid, contrary to the 

 findings of Baer and Blum, has no influence whatsoever either on 

 the sugar or on the nitrogen elimination. 



Another experiment was performed on a normal starving 

 animal. It received 10 gm. of glutaric acid without showing any 

 effect on the nitrogen elimination. 



Baer and Blum report that a good many compounds containing 

 two carboxyl groups possess the power of reducing the sugar and 

 nitrogen elimination. Experiments are in progress to verify their 

 contention. 



