Growth of Sarcoma in the White Rat. 57 



and in only six animals, or eighteen per cent., did the tumor grow, 

 and then only to a small size. The same number of controls were 

 inoculated with seventy per cent takes. 



To study to what extent anemia was present in these animals, 

 several animals were killed, the blood washed out of both extremi- 

 ties by salt solution, subsequently injected with bismuth, and 

 skiagraphed. These show that there is a marked difference be- 

 tween the vascular supply of the anemic leg, compared to the 

 normal one. 



Another set of experiments was performed to study the effect 

 of passive hyperemia of the leg, induced either by rubber ligature, 

 or by a bandage of adhesive plaster strips. A few hours after- 

 wards, the leg became slightly swollen, grayish red, and the 

 arteries still pulsating. This was continued for five to eight days, 

 caution having been taken not to permit edema. Twenty-four 

 hours after the onset of the hyperemia, twenty-five animals were 

 subcutaneously inoculated in the leg with Ehrlich's rat sarcoma, 

 and here the takes were ninety-six per cent. ; and the tumor grew 

 more rapidly and to a larger size than in the control animals, in 

 which the takes were sixty per cent. 



It is noteworthy that the anemic animals were subsequently 

 inoculated subcutaneously and in only two out of twenty-nine 

 did a growth result. It would seem, therefore, that these animals 

 possessed an acquired immunity, probably from the absorption of 

 substances from the first inoculation. 



From these experiments it becomes apparent that partial 

 anemia and passive hyperemia of the leg exert different influences 

 upon the growth of the transplanted tumor, similar to the dif- 

 ference noted by Moreschi in his experiments of over and under 

 feeding of his animals. 



Goldman, in his experiments, has shown that the blood supply 

 and the new formation of blood vessels are essential factors in the 

 growth of a cancer cell. 



Carl Levin, Bashford, and Gierke, have pointed out that the 

 fibro-plastic and angeoplastic reaction on the part of the host are 

 the deciding factors in the growth of a transplanted tumor cell, 

 and it shall be the aim of subsequent experiments to further study 

 the relation of the angeoplastic reaction on the part of the host 

 in the anemic and hyperemic condition. 



