94 Scientific Proceedings (48). 



ducing precipitins intra-peritoneally on successive days. Such a 

 method apparently gave rise to a potent precipitating serum and 

 they claim equally successful results in producing hemolysins. 

 These results as regards hemolysins were not confirmed in a later 

 communication by Bonhoff and Tsuzuki. 1 



We have found that one can obtain uniformly a sufficiently 

 potent hemolytic serum for fixation tests by immunizing rabbits 

 with washed sheep blood in a dose of one to two cubic centimeters 

 administered intravenously on three successive days. The hemo. 

 lytic titer of such a serum four days after the third injection is 

 usually 1 to 2,000. In other words, it is possible to produce a 

 thoroughly reliable hemolytic serum in one week by this method. 



Further details of this intensive method of immunizing to 

 produce hemolytic sera and also its use in producing precipitins, 

 bacteriolysins, agglutinins, and antitoxins will form the basis of 

 a more extensive communication to be published in the Patho- 

 logical series of the University of California Publications. 



68 (677) 



Note on the effect of the internal secretions upon the secre- 

 tion of epinephrin. 



By ISAAC OTT and JOHN C. SCOTT. 



One 2 of us was the first to show that the adrenal secretion 

 relaxed and inhibited the rhythmic contractions of the intestine. 

 Hoskins has shown that a dilution of 1-400 millions of epinephrin 

 inhibits the rhythmic contractions. It thus becomes the most 

 sensitive test for the presence of epinephrin. We tested its pres- 

 ence by the Magnus method. We injected a few grains of the 

 filtered solution of the different glands into the jugular of the 

 narcotized cat, and drew off, as Cannon has done, some blood from 

 the vena cava above the openings of the adrenal veins, and de- 

 fibrinated it. Normal blood defibrinated was then applied to 

 a segment of the intestine of a narcotized rabbit and its rhythmic 

 movements recorded. Then the blood, after the injection of the 

 glandular filtrate, was applied to the same intestine and its move- 



1 Bonhoff and Tsuzuki, Zeitschrift fur Immunitatsforschung., IV, page 180. 

 * Medical Bulletin, 1897, p. 376. 



