Pancreatic Transplantation in the Spleen. 35 



be instrumental in the removal from the blood of substances 

 present there in excess. In the experiments I wish to communicate 

 here, I selected, therefore, the bladder for the study of this problem 

 since the mucous lining of this organ is practically the only mem- 

 brane which possesses no glands. The rabbit was the experimental 

 animal used. 



After performing a double nephrectomy and tying a cannula 

 in the bladder, seven to ten grams of dextrose per kilogram were 

 injected intravenously in from twenty to fifty minutes, washing 

 the bladder several times during and after the injection. The 

 washings were then analyzed. The results are briefly as follows. 

 In nine experiments either no sugar or else indeterminable amounts 

 were found, and in four experiments only six to eleven milligrams, 

 i. e., 0.05 to 0.08 per cent, of the amount injected. In addition, 

 in some of the experiments uranine, the sodium salt of fluorescein, 

 was dissolved in the injection fluid. In the experiments in which 

 twenty milligrams were introduced, no trace of uranine, a very 

 diffusible dye, could be detected in the washings of the bladder. 

 In four experiments 100 milligrams were introduced; in two there 

 was not a trace and in the other two there was a barely perceptible 

 trace. 



The conclusion may therefore be drawn that the mucosa of the 

 bladder is practically impermeable for diffusible substances present 

 in the blood in great excess, even in the absence of the kidneys, 

 the chief organs of elimination of the body. 



25 (72i) 



Pancreatic transplantations in the spleen. 



By Joseph H. Pratt, of Boston, and Fred T. Murphy, of 



St. Louis. 



[From the Laboratory of the Theory and Practice of Physic, Harvard 



University.] 



The influence of pancreatic transplants in the spleen on the 

 prevention of diabetes was studied. The spleen was selected 

 because it has been shown that transplanted bits of tissue are 

 especially well nourished in the pulp of this organ. In nine 

 animals, bits of the pancreas of various size were buried in the 



