7 6 



Scientific Proceedings (52). 



selected solely with reference to the lowest and highest total 

 hemoglobin concentration in each series. Four entirely different 

 methods were employed and they all lead to the same result. 



Absorption of CO. 



654 

 658 



3-59 

 4.86 



Table I. 



Hemoglobin spectrophotometrically. 

 CO 

 Hb 

 I.29 



1.30 Laked ox blood. 



668 

 616 



2.88 

 5-40 



1.29 

 1.27 



Laked human blood. 



II. 



Reduction with palladium-hydrogen. Hemoglobin gravimetrically. 



k 



156 1.23 1.09 



154 11.50 1.08 Oxyhemoglobin 



blood. 



from 



Oxygen capacity by ferricyanide method. Hemoglobin spectrophotometrically. 



°i 

 Hb 



III. I.64 I.37 



4.27 1.40 



Spectrophotometric. 



Laked rabbit blood. 



log 



IV. 



Dilution 



2 

 10 



i-5 

 10 

 200 



1. 12 

 113 

 I.I3 

 1. 13 

 113 



Laked ox blood. 

 Laked ox blood after extrac- 

 tion with ether. 



log £7 



log 



1. 14 

 7.00 



A' 

 1.13 

 1.13 



Oxyhemoglobin from ox 

 blood. 



The first group of experiments was carried out with laked ox 

 blood and with laked human blood. The volume of CO absorbed 

 by oxygen-free blood ((NH 2 )2.H 2 0 and vacuum pump) was deter- 

 mined. The quantity of CO physically absorbed was deducted 

 from the total volume, using the coefficient of absorption for CO 

 in H 2 0 at the temperature of the experiment (20 0 C.) for the 

 calculation. Total hemoglobin concentration determined spectro- 

 photometrically. It will be seen from the table that for a small 



