102 



Scientific Proceedings (52). 



mining the amount of sugar Munson and Walker's "Uniform 

 method of sugar determination" was used and gave consistent 

 results. In order to test its efficiency the method was controlled 

 by division of a sample of blood into two portions. In one of 

 these the glucose was estimated directly. To the other a known 

 amount of glucose was added and then this amount was subtracted 

 from the total recovered, leaving a remainder which should equal 

 the amount found in the portion estimated directly. The results 

 of one such control are shown in Table I. 



Table I. 



Blood sample 6 (two cats). Total blood used 100.05 gm. 



Gm. blood in sample 



Gm. glucose recovered 



Gm. glucose added 



Gm. glucose in blood 



Gm. glucose per 100 gm. blood 

 Average for 06 and cd 



47-51 

 0.0314 



0.0314 

 0.0661 



47.51 

 0.0328 



0.0328 

 0.0690 

 0.0676 



47-51 

 0.0501 

 0.0178 

 0.0323 

 0.0680 



47-Si 

 0.0497 

 0.0178 

 0.0319 

 0.0671 

 0.0676 



It was necessary next to determine the amount of sugar 

 in the blood of normal cats. The results for a series of ten cats 

 are given in Table II. It will be noticed that with the exception 

 of the cat which gave 76 mgm. — obviously an anomalous case — 

 the greatest variation from the average is 4 mgms. The starred 

 numbers indicate controls such as are reported in Table I. The 

 results are calculated to grams of glucose per hundred grams of 

 blood. 



Average 



: II. 



L Table III. 



0.0676 •» 



0.1026 



0.0676 J 



0.0783 



0.0618 



0.0719 



0.0760 



0.0811 



0.0653 



0.0692 



0.0628 





0.0627 





0.0646 1 # 





0.0646 / 





0.0691 * 





0.0662 





The extracts of the pancreas that were used in the following 

 experiments were prepared as reported previously, except that the 



