Blood Pressor Substances in Immunity. 103 



temperature was at all times kept below 50 0 C. instead of being 

 allowed to go to 65 0 as before. So far five cats have been injected 

 with the extract, the first three with two injections each and the 

 others with one each. All injections were made beneath the skin 

 of the back. The results are shown in Table III and are calculated 

 to grams of glucose per hundred grams of blood as in Table I. 

 The average for the injected cats is over 21 per cent, above that 

 for the normal ones, and moveover the amount of sugar is greater 

 in each injected cat than for any normal animal except the one 

 that gave 76 mgm. This increase is very surprising and of peculiar 

 interest. At present I am not willing to venture any explanation. 

 There are however several possibilities which are amenable to 

 experiment and I hope that further work will throw some light 

 on it. In any case it would seem to put in grave doubt the idea 

 that the pancreatic hormone always tends to increase the storage 

 of glycogen in the liver at the expense of sugar in the blood. 



There are a number of factors entering into the experiments 

 so far performed that might cause the individual variations in the 

 experimental results. Some of the more probable are: the length 

 of time intervening between the injection and the death of the 

 animal; the amount of extract injected; the age of the extract; 

 the number of injections and the time intervening between them, 

 etc. At present I am trying to find some of the optimum condi- 

 tions. 



70 (766) 



On some blood pressor substances and adrenal separations 

 in experimental immunity. 



By J. P. Atkinson and C. B. Fitzpatrick. 



One of us in a work 1 on "The Preparation of Diphtheria 

 Antitoxin" endeavored to demonstrate by charts of the systemic 

 reaction following injections of cultures of the bacillus of diphtheria 

 and its toxin that the real crux of the process of immunization was 

 to determine when to re-inject. This question is still unsettled; 

 in short, of two animals treated the same, upon being re-injected 

 the one, which may apparently be the better prepared, dies and 

 the other recovers. 



•Fitzpatrick, N. Y. Medical Journal, April 27, 1895. 



