104 



Scientific Proceedings (52). 



This aspect of immunization is very well brought out in the 

 following example. 



Romer and Joseph 1 re-infected two tuberculous sheep with 

 a culture of which 1 mg. per 10 kg. killed healthy sheep in one 

 month. One of these sheep was re-infected with 1 mg. per 10 kg. 

 ten months after a previous inoculation with a .2 mg. per 10 kg. 

 and 15 months after a first injection of .1 mg. per 10 kg. This 

 animal died in 48 hours. The other one of these sheep was re- 

 infected with the same dose (1 mg. per 10 kg.) ten months after 

 previous injection of .2 mg. per 10 kg. This animal responded 

 with an intense reaction. The reaction was followed by a return 

 to health. 



We have shown in studies read before this society that the 

 blood of tuberculous animals especially when about to die of 

 tuberculosis contains a depressor substance. The use of this 

 blood in conjunction with tuberculin was likewise shown to give 

 an effective immunity against fatal infection with the B. tubercu- 

 losis in one case, and in some others the fatal ending was delayed 

 beyond the controls. It was also demonstrated that the injection 

 of this serum shortly after the injection with the tuberculin 

 rapidly caused death. We have also found depressor substances 

 to be present in the blood serum of other diseases. 2 



The first part of our present report consists of results of the 

 injection of blood serum obtained from animals recovering from 

 inoculations of B. tuberculosis and the possible application of these 

 observations to the practical therapy of infection and intoxication. 



Protocol 3 April 19, 1912. — A 14 lb. female dog, sensitized 

 April 18, 1912, with % c.c. of crude tuberculin. 



8 c.c. of the serum of a calf recovering from repeated doses of 

 culture of B. tuberculosis (human type), when injected into the 

 femoral vein of this dog gave a decided rise in blood pressure. 

 This rise was preceded by a very slight depression. This dose 

 was repeated in six minutes with a like result. Five minutes later 

 the same dose was injected and gave no response. 



'"Die Tuberculose Re-infection," Beitrage zur Klinik der Tuberculose, 1910, 

 XVII, pages 330-333- 



2 Atkinson & Fitzpatrick, Proc. Soc. Exp. Biology and Medicine, 1910, VII, 

 PP- 77-79; ibid., pp. 104-107; ibid., VIII, pp. 24-28; ibid., pp. 49-5L 1912. 



3 3 c.c. normal calf serum gave no response when injected into the femoral vein 

 of dog sensitized with tuberculin. 



