Influence of Pancreatic and Duodenal Extracts. 173 



the reduced elimination of that substance is proved by the following 

 experiment on another dog place in the respiration calorimeter. 



Dog "5." Operated May 6. 



Date. 



Time. 



Glucose 

 in urine 

 per 

 hour. 



Nitro- 

 gen in 



urine 

 per 



hour. 



D:N. 



co s , 



gm. 



O s . 

 mg. 



R.Q. 



Heat 

 pro- 

 duced. 



Temp, 

 of dog. 



5/9/13 



9-4S-I0.4S 









8.62 



9.22 



0.68 



21-73 



38.6 





10.45-11-45 









9-97 



10-55 



0.69 



27.12 



38.6 





8. 50-11. 55 



1-74 



O.56 



308 















P.M. 





















12.35- 105 



150 c.c. exts. of pancreas and duodenum from 2 normal dogs 







injected intravenously. 20 gms. glucose given per os. 





11.55- 1.05 



1.07 



0-39 



2.70 















1-55- 2.55 









13.60 



14-13 



0.70 





39-6 





2-55- 3-55 









11.84 



11.78 



0.73 









3-55- 4-55 









10.16 



10.71 



0.69 





39-9 





1.05- 5.05 





0.45 















Already in the first hour's urine, including the period of 

 injection, a marked decline in the sugar elimination is seen, showing 

 that the typical effect on the excretion of sugar would have been 

 produced, if glucose had not been fed. With 20 grams of glucose 

 available, however, none, or an extremely small quantity at the 

 most, was burned. The increase in respiratory metabolism the 

 first two hours was due to restlessness of the dog. 



Other experiments after injection of pancreatic extract alone 

 and after injection of normal dog's blood likewise showed no 

 effect on the respiratory quotient. 



Incidentally it has been found in the single experiment in the 

 respiration calorimeter that the heat production in the depan- 

 creatized dog was from 30 to 50 per cent, higher than the normal 

 on the same dog determined one month earlier. This confirms 

 the observations of Benedict and Joslin in severe cases of human 

 diabetes. 1 



1 Benedict and Joslin, Carnegie Institution of Washington Publication No. 176. 



