Re-injection with B. tuberculosis. 



19 



Control: Small white dog, 12 lbs., received April 25, 1912, an 

 initial injection of 4 mg. of bovine culture No. 634, intravenously 

 in 4 c.c. of physiological saline solution. The dog was sick and 

 lost weight for several months, and then regained its weight. Two 

 superficial abscesses with necrosis of the skin and subcutaneous 

 tissue developed at the point of inoculation, which healed after 

 a few months. This dog was re-injected intravenously April 16, 

 1913, with 4 mg. of a virulent bovine culture (Ravenel) of the B. 

 tuberculosis contained in 4 c.c. of physiological saline solution. 

 The healed ulcers of the initial injection re-opened 12 days after 

 the re-injection as two clean, punched out ulcers and an area of 

 infiltration arose at the site of the re-inoculation. This dog 

 became much emaciated and had a purulent conjunctivitis. No 

 hemorrhage was observed from nostrils or in stools. Respiration 

 48 per minute, 19 days after the re-injection. Died May 12, 1913. 

 Autopsy showed a typical general miliary tuberculosis, with 

 mediastinal glands enlarged and caseous. The cause of death in 

 this dog was undoubtedly general tuberculosis. 



Protocol 2: Controls on Culture No. 634 (Bovine). 



Mongrel dog, 25 lbs., injected intravenously April 25, 1912, 

 with 4 mg. No. 634 bovine culture of B. tuberculosis, was killed 

 June 26, 1912. The autopsy showed a case of typical infection. 

 The lungs contained numerous small tubercles, with no caseation 

 or consolidation. Kidneys, about 300 large tubercles. Liver, a 

 few tubercles. Several other dogs were used to control the bovine 

 cultures, Ravenel and No. 634. 



Protocol 3: Delayed Anaphylaxis. 



A small quantity of the insoluble non-toxic portion of the 

 tubercle bacilli, prepared according to Vaughan's method, was 

 injected into each of 2 guinea pigs. After 3 weeks, these two pigs 

 were re-injected, intraperitoneally, with an emulsion of tubercle 

 bacilli, from which the split products had been prepared. 



Pig No. 1 showed some restlessness, at the time of re-injection, 

 but gave no other sign that it had been affected by the re-injection. 

 Two days later this pig was seized with a convulsion and died. 



Control Pig No. 2: Re-injected as above. 30 minutes after 



