20 



Scientific Proceedings (55). 



the re-injection, restlessness followed by weakness in the hind legs; 

 violent convulsions followed by extreme fatigue. The convulsions 

 continued and death followed in a convulsion six hours after the 

 injection. 1 



Protocol 4: Re-injection with Tuberculous Serum 

 following a tuberculin injection. 



The subcutaneous injection of 10 c.c. of the serum of a tuber- 

 culous rabbit into each of three rabbits, each of which had been 

 previously sensitized by an injection of 1 c.c. of crude tuberculin, 

 caused death in 24 to 50 hrs. 2 



The autopsy showed, as the principal lesion, large, marked 

 areas of intestinal inflammation and necrosis. 



If this state is dependent on substances with free bonds or not 

 homogenized, or on split products, as we have outlined, one should 

 be able to detect it by the presence of reactions, changes of blood 

 pressure, precipitins, ferments or similar agents, loose in the fluids 

 or stored in the organs. 



It has been shown by one of us that tubercle bacilli suspended 

 in a saline solution are considerably changed upon being elec- 

 trolyzed in their reaction to stains. If a porous cell such as a 

 Berkfeld filter is used as a septum in a U-tube and suspensions of 

 tubercle bacilli are put in both arms, one portion will be acted 

 upon by chlorine and the other portion by sodium or sodium 

 hydrate as a result of electrolysis. The organisms acted upon by 

 chlorine lose their acid-fast quality when decolorized by nitric 

 acid and substances that have the acid-fast principle may be 

 shaken out of solution with ether. The portion acted upon by 

 sodium or sodium hydrate does not noticeably lose its acid-fast 

 principle. There are other changes which can be demonstrated 

 and which are now being investigated. 



A guinea pig was re-injected twice with tubercle bacilli which 

 had been submitted to the above chlorine treatment. The injec- 

 tions were made during a period of two weeks. Within an hour 

 after the third injection the guinea pig showed signs of illness and 

 became steadily worse during the day. It died during the night 



1 A. and F., Proc. of Soc. for Exp. Biol, and Med., 1910, VIII, pp. 24-28. 



2 A. and F., Proc. of Soc. for Exp. Biol, and Med., 1910, VII, pp. 77-79. 



