J 34 



Scientific Proceedings (59). 



of nitrogen, half of which was given as urea, throughout a uranium 

 nephritis without any retention.) This would seem to indicate 

 that the increase in non-protein nitrogen of the blood in such 

 animals is due in part to an abnormal chemical combination 

 which can not pass the kidney and is not necessarily due to 

 impaired kidney function. 



Before ascribing an increase in the non-protein nitrogen of 

 the blood in any form of nephritis to kidney insufficiency, the 

 influence of all the above factors should be taken into account. 



83 (900) 



The influence of induced diabetes on malignant tumors (including 

 a report of a case of human phlorhizin glycosuria). 



By Stanley R. Benedict and Robert C. Lewis. 



[From the Department of Chemistry, Cornell University Medical 

 College, and from the Research Laboratory of General 

 Memorial Hospital, New York City.] 



Experiments have been reported by Beebe and Van Alstyne 

 showing that withdrawal of carbohydrates from the diet of white 

 rats markedly inhibits the "taking" and rate of growth of trans- 

 plantable malignant tumors in these animals. Cremer and 

 Lockhead have recently shown that carbohydrate is utilized in 

 the growth of tumor tissue in experimental tumor rats. As a 

 development of the idea that utilization of glucose plays an im- 

 portant role in the synthesis of new protoplasm, we have carried 

 out experiments upon rats planted with the "Buffalo sarcoma" 

 in which the animals were placed upon a carbohydrate-free diet, 

 and at the same time rendered diabetic through the injection of 

 0.2 gram doses of phlorhizin in olive oil once in two or three days. 



As a brief summary of our results in this connection it may be 

 stated that we have worked with about forty rats in all, so far, and 

 that the results are so striking and constant as to warrant a 

 positive statement that the production of the complete diabetes 

 in experimental sarcoma rats is followed by retrogression and 

 ultimate cure of the growth in every case where the growth at the 



