Mammals from Ancestors Treated with Alcohol. 137 



turity are usually nervous and slightly under sized. These animals 

 Fi, or the second generation, are never themselves subjected to 

 the fume treatment. Mated even with normal animals the 

 results are poor when compared with the outcome of normal 

 control matings. Twenty-six matings of second generation with 

 normal animals gave in 4 cases negative results or early abortions, 

 2 stillborn litters of 4 individuals and 20 living litters containing 

 31 young, 19 of which died and only 12 survived. Twenty-two 

 matings of second generation by alcoholized animals gave 5 

 negative results or early abortions, 3 stillborn litters of 7 young 

 and only 14 living litters consisting of 25 young, n of which died 

 and 14 survived. 



If second generation animals, non-relatives in most cases, are 

 mated with one another the sum total of the result is worse than 

 when they are mated in either of the above combinations. Forty- 

 seven such matings gave in 14 cases negative results or early 

 abortions, 3 stillborn litters containing 8 young, and only 30, less 

 than 64 per cent., living litters consisting of 46 young; 14, or 

 about 33 per cent., died soon after birth and 32 survived. 



A point of interest is that several of these offspring, F 2 , or third 

 generation, show gross defects or deformities. Only two of the 

 immediate offspring from alcoholized animals have shown a 

 clouded condition of the cornea of one eye, and no defect or 

 deformity of any nature has been observed in 89 control young. 

 However, among the 54 F 2 , or third generation, young that have 

 reached term 9 or about 17 per cent, show gross eye defects. Two 

 had opaque corneas, 3 complete cataracts in both eyes, the lens 

 being milk white, 2 had one normal eye while the other was about 

 half size and blind, one has only one eye, that of the opposite 

 side being completely absent, and finally one animal was entirely 

 eyeless, having no indication of eyeballs, optic nerves or chiasma. 



These abnormal animals arose from parents that had not been 

 subjected to the alcohol treatment, although in all cases two o r 

 more of their grandparents, and usually only the paternal ones 

 had been treated for various lengths of time with the fumes of 

 alcohol. 



Only a few matings of the third generation, F 2 , animals have 

 been made, yet if conclusions may be based upon these small 



