SCIENTIFIC PROCEEDINGS. 



Abstracts of Communications. 



Sixtieth meeting. 



Carnegie Laboratory for Experimental Evolution, Cold Spring Harbor, 

 June 6, IQ14. President Lusk in the chair. 



98 (915) 



Extra sugar during ether and nitrous oxide narcosis in fully 

 phlorhizinized dogs. Sources of error in existing methods 

 for the study of gluconeogenesis. 



By W. D. Sansum and R. T. Woodyatt. 



[From the Otho S. A. Sprague Memorial Institute Laboratories for 

 Clinical Research, Rush Medical College, Chicago.] 



Dogs were prepared by injecting subcutaneously 1 gram of 

 phlorhizin suspended in olive oil once every twelve hours until 

 the G : N ratio was constant, then every 24 hours. 



It has been observed by Lusk 1 that fasting and phlorhizin alone 

 do not remove all glycogen from the body. This was confirmed 

 for dogs prepared in the manner described. Such animals were 

 narcotized with ether, nitrous oxide and other narcotics. Narcosis 

 with ether and nitrous oxide was always followed by a large increase 

 of the glycosuria with a rise in the G : N ratio. When ether and 

 chloroform were used, there was as a rule, a fall in the output of 

 nitrogen and acetone bodies and a fall in sugar following the initial 

 rise, but with nitrous oxide the rise in sugar was unaccompanied 

 by such changes. In one of the ether experiments also the fall in 

 nitrogen was very slight and there was no lessening of the gly- 

 cosuria following the initial increase. 



An animal prepared in the usual way was given a cold bath for 

 20 minutes followed by 6 hours of moderate shivering. After this 

 I mg. of epinephrine was given intravenously once every 6 hours. 



1 Ergeb. der Physiol., XIII, p. 361 (1912). 



157 



