i8o 



Scientific Proceedings (60). 



hi (928) 



Specificity of the complement deviation test in experimental 



tuberculosis. 



By J. Bronfenbrenner. 



[From the Laboratories of Western Pennsylvania Hospital, Pittsburgh, 



Penna.] 



At the meeting of this Society on February 18, I reported the 

 results of my preliminary attempts to prove the specificity of 

 complement deviation in tuberculosis with Besredka's tuberculin, 

 in some cases giving positive Wassermann reaction at the same 

 time. 



In this paper I would like to present the results of my experi- 

 ment proving the possibility of independent existence of the two 

 reactions in the same animal. 



I produced an experimental orchitis in a series of rabbits, and 

 on March 3d selected amongst them the animals giving positive 

 W.R. All the animals were tested for Tb.R. but it was invariably 

 negative. 



When, however, infected with tuberculosis, part of these 

 animals developed Tb.R. For a time both reactions were existing 

 until W. R. disappeared in some animals spontaneously, in others, 

 as shown on the table under the influence of salvarsan administered 

 intravenously (0.04 gram per kilo), the Tb.R. persisting. The 

 table on page 181 is the protocol of the experiment. 



112 (929) 



One hundred parthenogenetic generations of Daphnia without 



sexual forms. 



By Arthur M. Banta. 



[From the Station for Experimental Evolution, The Carnegie In- 

 stitution of Washington.] 



November 17, 191 1, the writer began rearing a number of pure 

 lines of Daphnia pulex from large females taken in out-door ponds. 

 The females were reproducing parthenogenetically and no males 

 or "winter" eggs were found in the pond. 



