Trikresol in Subdural Injections of Sera. 183 



The records were taken with the kymograph, the pressure being 

 taken at the carotid artery. The injections were made into the 

 vertebral artery, the femoral artery and vein, the carotid artery 

 and into the spinal canal. 



The results which are somewhat contradictory, were as follows: 



An antimeningococcus serum which had produced rashes and 

 other disturbances in patients, caused well-marked depressions in 

 6.5 c.c. doses. Preservatives were chloroform and 0.4 per cent 

 trikresol. The injections were made into the femoral vein. A 

 whole antipneumococcus serum preserved with chloroform tested 

 in the same way produced death. 1 



Experiments (by F. and A.) with antimeningococcus serum to 

 which was added varying quantities of trikresol (from .1 per cent 

 to .4 per cent) gave no deleterious results when first mixed, but 

 after standing one week in some cases depressions were obtained. 



An injection of 2 c.c. of antimeningococcus serum prepared 

 19 days before the experiment by the addition of .4 per cent 

 trikresol was made into the vertebral artery. A marked depression 

 resulted. Six c.c. of this serum also injected into the vertebral 

 artery caused immediate clotting and were followed by a con- 

 vulsion with almost complete cessation of respiration. 



Further experiments carried out (by F., A. and Z.) on six 

 dogs gave the following results: 



1. As a rule, there was apparently no marked disturbance of 

 blood pressure in normal dogs immediately after the lumbar sub- 

 dural administration 2 by gravity or careful gentle pressure of 

 moderate doses of antimeningococcus sera containing .3 per cent 

 trikresol. 



2. Similar injections were made without marked disturbance 

 in blood pressure of physiological saline solution, "old" antimenin- 

 gococcus serum, antimeningococcus serum containing .3 per cent 

 and .4 per cent trikresol, plain normal horse serum, normal horse 

 serum containing chloroform, antistreptococcus serum containing 

 chloroform and antipneumococcus serum containing chloroform. 



3. Pressure appears to be a factor of real danger. 



•Vide, "Some Vaso-reacting Substances in Blood Serum," Proc. of Soc. for 

 Exp. Biology and Medicine, 1912, LX, pp. 49-51. 



2 Lumbar puncture with skin incision through the skin and muscles was em- 

 ployed. Laminectomy was not used. 



