Cholesterol Content of Blood Serum. 25 



This removal was performed in two stages. First, the entire 

 large intestine was separated from the mesentery by means of an 

 abdominal incision, and the abdomen closed. The entire colon 

 was then withdrawn through the anal opening, by a modified 

 Whitehead operation, care being taken not to injure the anal 

 sphincters. The end of the ileum was sutured to the anal mucosa- 



After the operation, the dogs were kept on a milk diet for 

 about a week, and then placed on an ordinary mixed diet. Most 

 of the dogs died from shock or intercurrent infections, or were 

 killed for pathological study at the end of from one to two weeks. 

 One dog, however, was kept for three-and-a-half months. 



This dog showed a rapid loss of weight during the first two 

 weeks following the operation, after which it slowly gained in 

 weight till the end of the experiment. The dog apparently suf- 

 fered no inconvenience from the operation, other than that from 

 the frequent passage of semi-liquid stools. 



At autopsy this dog showed no pigmentation of the internal 

 organs that could be detected macroscopically. Frozen sections 

 and celloidin sections of the spleen, liver, pancreas, small intestine, 

 kidney, bone-marrow and heart muscle showed no pigment de- 

 posits. No iron-containing pigment could be detected in these 

 organs by the Berlin blue reaction. 



The total removal of the large intestine in dogs, therefore, 

 apparently does not produce a recognizable degree of haemo- 

 chromatosis within a period of three-and-a-half months. 



Experiments extending over a longer period of time will 

 be reported later. 



15 (1597) 



Variations in the total cholesterol content of the blood serum in 

 pernicious anaemia and pneumonia. 



By H. A. KiPP (by invitation). 



[From the Laboratory of Pathology, University of Pittsburgh.] 

 Pernicious anemia and pneumonia are pathological states 

 in which the cholesterol content of the blood is known to be 

 altered during the course of the disease. In pernicious anemia, 

 the cholesterol content of the serum is depressed to a varying 



