34 Scientific Proceedings (109). 



difficult, but in the four animals that we have injected in this man- 

 ner and which have died or been killed in from one to five days, 

 definite consolidation of the lungs was evident in all and a sero- 

 fibrino-purulent pleurisy occurred in all but the 24-hour case. 



A histology study of the lungs in these cases apparently shows 

 the characteristics described by MacCallum in his interstitial 

 broncho-pneumonia, namely, plugging of aveoli with polymor- 

 phonuclear leucocytes, red blood corpuscles, serum and fibrin in 

 definite relation to bronchi which are also filled with a purulent 

 exudate. There is a definite infiltration of polymorphonuclear 

 leucocytes and lymphocytes about the bronchi and blood vessels 

 and marked desquamation of the bronchial epithelium. A further 

 study will show in what respect, if any, this experimental pneu- 

 monia in rabbits differs from that produced by the pneumococcus. 



22 (1604) 



The bactericidal action of rabbit bile on certain strains 

 of streptococci. 



By Ruth L. Stone (by invitation). 



[From the Department of Pathology and Bacteriology, University of 

 California, Berkeley, California.] 



The phenomenon here described was noted during the course 

 of a series of experiments on rabbits designed to test the patho- 

 genicity of a certain strain of hemolytic streptococcus. It was 

 found that, although at autopsy the various organs of the peri- 

 toneal cavity were filled with living streptococci, the bile was 

 always sterile. This led to the testing, in vitro, of bile from other 

 rabbits as well as from various other animals, to find out, whether 

 they possessed bactericidal action on this strain of streptococcus. 

 All samples of rabbit bile proved to be bactericidal, whereas the 

 bile of the ox, sheep, cat, dog, pig, guinea pig, and human exerted 

 no deleterious effect on the streptococci. 



The strain of streptoccus used (Strain "H") 1 in these prelimi- 

 nary experiments was, according to Holman's classification, 

 Streptococcus pyogenes — a hemolytic, non-mannite fermenting strep- 



1 Gay and Stone, J. Infec. Dis., 1920, xxvi, 265. 



