58. 



Scientific Proceedings (iio). 



active anaphylaxis in the lower monkeys against horse serum, and 

 found that the serum of the lower monkeys did not sensitize 

 guinea pigs passively. His horse serum injections produced no 

 effects in the monkeys, but in his report there is no analysis of 

 antibody production or other details which would permit one to 

 draw one's own deductions. 



As a necessary accompaniment to some other work we were 

 engaged in, we thought it desirable to do a few experiments, 

 worked out in careful detail, on the problem of antibody production 

 and anaphylaxis in the lower monkeys, and we used, for this 

 purpose, two Macacus rhesus, I Macacus cynomolgus, and 2 South 

 American ringtail monkeys which happened to be available in the 

 laboratory at different times. 



Experiment I. 



The first experiment was carried out in order to determine by 

 the most delicate test available to us, namely, the isolated uterus 

 method of Dale, whether or not a single injection of horse serum 

 would produce any degree of hypersensitiveness in a monkey, 

 analogous to that produced by such injections in guinea pigs. 



For this purpose this monkey, Macacus cynomolgus, was 

 etherized on March 13, 1920, and the right fallopian tube removed, 

 and mounted in the Dale apparatus, in a 200 c.c. Ringer solution 

 bath. The monkey was sewed up aseptically, and given 3 c.c. 

 of sterile horse serum intravenously after the operation. 



After the fallopian tube had begun to show regular rhythmic 

 contractions, as shown in the chart, 1 c.c. of horse serum, diluted 

 in 10 c.c. of Ringer's, was introduced into the bath, and this 

 process was repeated twice, at about five-minute intervals, in 

 order to make sure of the quantities of horse serum which could 

 be given without eliciting reaction in the uterus of the untreated 

 or normal monkey. 



In the interval between March [3 and April 27, intracutaneous 

 skin reactions with 0.2 c.c. of a 1 — 1 5 horse serum dilution were 

 done on March 22, March 30. April 14, and April 22, and titrations 

 of monkey serum were made on the same days, against horse 

 serum, and against anti-horse serum, to determine the formation 

 of antibodies, or the persistence of antigen, respectively. In no 



