Anaphylaxis in Lower Monkeys. 



59 



case was a definite skin reaction obtained, and in no instance was 

 any precipitating antibody discovered. On April 27, the monkey 

 was again etherized and the other fallopian tube removed by 

 operation. The monkey was again sewed up and the rem'oved 

 tube placed in the Dale apparatus as before. The same horse 

 serum instillations were made, again without the slightest effect, 

 as shown by the attached records (see Fig. 1). 



At the same time, the monkey, as soon as he came out of the 

 ether, was reinjected with 3 c.c. of horse serum, intravenously, 

 without showing the slightest reaction that could be interpreted 

 as anaphylaxis, either immediately or later. 



This experiment shows definitely that a single injection of 

 horse serum did not elicit antibody formation or anaphylactic 

 symptoms in a Macacus Cynomolgus. 



Experiment II 



Monkey II, Small Cebus Monkey. — On March 15 received 4 c.c. 

 horse serum intravenously. Skin reaction 0.1 c.c. of 1-5 dilution 

 of horse serum, intracutaneously, done just before injection, was 

 entirely negative. March 22, skin reaction, negative, 2 c.c. horse 

 serum intravenously, no symptoms, except slight shivering, two 

 soft defecations, tremor in the legs beginning 12 minutes after 

 injection ; perfectly well in 30 minutes. April 7, titration of serum 

 against horse serum shows a faint but distinct ring in the tubes 

 containing the horse serum, dilution of 1-2, and 1-10. In con- 

 sequence, on April 8, a skin test was done with 0.05 c.c. of 1-10 

 horse serum, which showed slight redness coming on within 3 

 minutes, surrounded by a small edematous area, which faded 

 out within 30 minutes, and was regarded by several observers as 

 distinctly different from other reactions produced on the monkeys. 

 This was interpreted as a mildly positive reaction. 



Immediately after the skin reaction, 1.5 c.c. horse serum was 

 intravenously injected. After the injection, seems uncomfortable 

 and abnormally quiet. After 30 minutes, marked redness of the 

 face, and the lower part of the face and lips are distinctly swollen 

 and edematous. This was noticed by several observers, and 

 checked up by our technician who handles these monkeys con- 

 tinuously. This lasted about 2 hours, when it gradually returned 

 to normal. 



