62 



Scientific Proceedings (iio). 



solution and filtering through moist paper. Before these monkeys 

 were injected, their blood serum was titrated to ascertain whether 

 there were non-specific cross reactions between monkey serum 

 and egg white, and no such reactions were obtained. Skin re- 

 actions and titrations were done at frequent intervals in the course 

 of the experiments, and were we to detail all of these, we would 

 make a long communication of a relatively simple matter, since 

 there are no technical difficulties in the methods used, sufficiently 

 familiar to all serologists. We will, therefore, omit protocols 

 and state the pertinent results briefly. 



Monkey III, Macacus Rhesus. — Preliminary titration showed 

 no reactions with egg white or with anti-egg rabbit serum. An 

 attempt was made to repeat the fallopian tube experiment with 

 this monkey, but owing to the relatively much deeper pelvis of 

 the rhesus variety and the astonishingly small fallopian tube of 

 this young female, the results, although negative, were not con- 

 vincing and will not be cited. 



May 24, 2 c.c., 1-5 egg white, intravenously. Intracutaneous 

 skin reaction before this injection, negative. 



June 1, no antibodies to egg white. 



June 17, skin reaction negative, intravenous injection of 10 ex., 

 1-5 egg white, no reaction. 



July 7, skin reaction negative; titration shows a very faint 

 positive precipitin reaction against egg albumin, 1-10, and anti- 

 egg rabbit serum shows very faint reactions up to dilutions of 

 monkey serum of 1-5. 



July 12, 10 c.c., 1-5 egg white, intravenously. Immediate 

 moderate respiratory distress ; irregular and rapid breathing within 

 2 minutes; staggers and turns about as though loss of equilibrium; 

 hind legs weak; holds onto cage; falls down twice, but immediately 

 up again; seems much distressed, but is practically well again in 

 5 minutes. All of these symptoms took place after monkey had 

 been left alone in cage and effects of handling could be excluded. 



July 27, titration, definite and fairly strong precipitin reaction 

 against egg white in dilution of 1-10. Anti-egg rabbit serum 

 shows no antigen in the monkey serum. Skin reaction on this 

 day, negative; 10 c.c. of 1-5 egg white injected, intravenously; 

 after 5 minutes rapid respiration, great pallor, falls to the ground 



