6 4 



Scientific Proceedings (iio). 



Since, in this monkey, as well as in the preceding ones, we 

 were never able to obtain any considerable amount of antibody, 

 and failed to find antigen in the blood within relatively short 

 periods after injection, we naturally wondered what became of 

 the egg white injected. For this reason we placed this last 

 monkey into a metabolism cage after the last injection, and within 

 2 hours after the injection of 14 c.c, 1-5 egg white, we obtained 

 through a filter-covered funnel, about 12 c.c. of clear urine. This 

 urine was light colored, and neutral to litmus, and was titrated 

 against anti-egg rabbit serum in various dilutions. It gave very 

 heavy and rapidly appearing precipitin reactions against anti-egg 

 rabbit serum in dilutions ranging from concentration to 1-10, 

 and distinctly and visible reactions in dilutions as high as 1-40. 

 Parallel titrations against anti-ox and anti-streptococcus rabbit 

 serum, carried out to make sure that we were not dealing with 

 any fortuitous property of the urine which would cause reactions 

 with rabbit serum in general, were entirely negative. 



Conclusions. 



In the preceding monkey experiments we have confirmed the 

 observations of a number of workers, especially of Berkeley, that 

 monkeys are very poor animals to use for antibody production 

 and, incidentally, observed that antihorse rabbit serum often 

 gives definite reactions with normal monkey serum. 



In the first experiment with a Macacus cynomolgus, carried 

 out by the separate testing of the two fallopian tubes by the Dale 

 method, it appeared that a single injection of horse serum pro- 

 duced not the slightest trace of an anaphylactic sensitization. 

 This was borne out by the results of intravenous injections into 

 the same monkey. No antibodies were formed in this monkey 

 as far as could be ascertained by the precipitin test. 



In Monkey II, a small Cebus monkey, and Monkey III, a 

 young female Macacus rhesus, small amounts of antibody were 

 formed after repeated injection, and moderate reactions, probably 

 of an anaphylactic nature, were obtained. 



In Monkey 1 1 , 1 7 days after the second injection of horse serum, 

 a small amount of antibody was found in the serum, and reinjec- 

 tion elicited a reaction which, it seemed to us, as justifiable to 



