86 



Scientific Proceedings (hi). 



It is to be seen from the above that of living cell masses from 

 eight different plants, five showed a greater hydration capacity 

 in the vitamine solution than in water, two were notably less 

 and one had a swelling capacity only slightly less than in water. 

 Of the five which gave excessive enlargement in a living state, 

 two were tested in mature condition and also found to show a high 

 capacity in the vitamine. 



Two of the five which showed excessive swelling in a living 

 condition, repeated this action in a dried condition. Lastly it 

 was found that of the three kinds of cell-masses which did not 

 give the normal hydration in vitamine, two gave an excessive 

 swelling in a dried condition. 



Coincidently various colloidal preparations were hydrated 

 in the vitamine solution and the swellings at 15 0 C. relative to 

 that in water are given below. 



Agar 140 



Agar and " salts " 73 



Agar and soap 132 



Agar and potassium oleate 80 



Agar 3, gelatine 2 136 



Agar and phenyl alanine 95 



Agar 3, gelatine 2 and "salts" 130 



Agar 3, gelatine 2, soap 92 



Agar 3, gelatine 3 135 



Agar 2, gelatine 3 143 



Agar 2, gelatine 3 and "salts" 130 



Gelatine 163 



Gelatine and "salts" 80 



Gelatine and soap 92 



The "salts" of the above mixtures consisted of 20 cc. KC1 at 

 0.001 M ; 12 cc. NaCl at 0.001M ; 2 cc. CaCl at 0.0001 M, added for 

 each gram of air dried material in making up the colloidal mixtures 

 in water before casting and drying. The soap was a well-known 

 brand sold in flakes and was used as one to a thousand in the 

 colloidal preparation to which it was added. The agar used 

 alone was a purified preparation by Dr. Harris, but bacto-agar 

 was used in the preparation containing soap. 



Finally a preparation of agar in which one thousandth part 

 of the amino-acid phenylalanine had been incorporated was 

 hydrated in the vitamine solution with results not essentially 



