Chronic Focal Lesions in Animals. 



121 



of propyl, allyl and cinnamyl alcohol, were tested regarding their 

 sperm-stimulating and fertilization promoting effects, and also 

 their susceptibility to oxidizing agents and other chemicals, and 

 were found to exhibit effects corresponding very closely with 

 those exerted by the egg secretions and extracts. The exact 

 constitution of the substance or substances present in the extract, 

 has not yet been determined on account of the paucity of material 

 at the end of the season, but it is proposed to continue this investi- 

 gation next summer. 



57 (1639) 



On a method of producing chronic focal lesions in animals. 



By HANS ZINSSER and EDWARD H. RAYMOND, Jr. 



[From the Department of Bacteriology, College of Physicians and 

 Surgeons, New York City.] 



Celloidin capsules are made by a method first used, we believe, 

 by Dr. Clarke of the College of Physicians and Surgeons, though 

 we are not sure of this, but certainly not entirely original with us. 

 Small balls or globes of sugar in the form of some of the more 

 commonly purchasable candies, are stuck to small silk threads 

 with a hot forceps. These are dipped three or four times in 

 celloidin, hardened for a short time in alcohol, and thrown into a 

 jar with running water. The sugar diffuses in the course of ten 

 or twelve hours, and a completely closed capsule is left. With a 

 fine needle a hole is punctured through the capsule, the water 

 drained out, and agar, inoculated with streptococci or other 

 organisms desired, is injected into the capsule and allowed to 

 harden. The puncture-hole is left open. The capsule is then 

 dropped into the peritoneal cavity of a rabbit and the rabbit 

 sewed up. In most cases the rabbits live for months. Some of 

 them gradually emaciate, others will develop agglutinins. We 

 have opened a number of rabbits from six weeks to four months 

 after the capsule had been placed into them. In one case a rabbit 

 into which the capsule had been placed in July was opened in the 

 middle of November (over four months) and the capsule was 

 found to contain living streptococci at this time. Apparently the 



