Scientific Proceedings (113). 



2. The cells of young embryonic tissue contain also a reserve 

 energy. They may grow in an atmosphere of pure nitrogen for 

 a short time. This absence of oxygen leads soon, however, to 

 their rapid disintegration. Oxygen is evidently necessary for 

 their preservation as Warburg conceived it. 1 



3. This ability for the cells to grow without oxygen is lost with 

 development. It fails in the case of the cells of older embryos; 



>x 



+ + fe?. v «i)C v 3-7.) voy&s&S 



— 



chick embryos, a^es 4x7 days. 



Table and Curve i. The Relative amount of growth in the cultures is indi- 

 cated by the number of + signs. That noted in the 4 day old embryonic tissue in 

 7.48 per cent, oxygen is comparable to what is seen in air. 



as development proceeds, a greater and greater amount of oxygen 

 is needed. 



63 (1645) 



Does growth require preformed carbohydrate in the diet? 



By THOMAS B. OSBORNE and LAFAYETTE B. MENDEL. 



[From the Laboratory of the Connecticut Agricultural Experiment 

 Station, and the Sheffield Laboratory of Physiological Chemistry 

 in Yale University, New Haven, Conn.] 



Carbohydrates are ordinarily regarded as indispensable com- 

 ponents of the food intake. This belief is based on the presence 



Ergeb. Phys., 1916, xiv. 



