222 



Scientific Proceedings (115). 



the tests. In general, complement fixation tests like the pre- 

 cipitin reactions, showed differences between the various prepara- 

 tions of Bence- Jones protein, a cross-reaction between human 

 serum and the salted-out specimens of non-crystallizable Bence- 

 Jones protein, and a complete difference between the crystalline 

 Bence- Jones protein and blood-serum. 



Anaphylactic Reactions, — It was difficult to sensitize guinea pigs 

 to the crystalline Bence-Jones protein, though not to the other 

 preparations, indicating again an antigenic difference in that 

 respect. Guinea pigs were sensitized actively by the intravenous 

 injection of 0.25 c.c. of a 6 per cent, solution of the various Bence- 

 Jones proteins, and by 0.25 c.c. of human serum. Some animals 

 were passively sensitized by the intraperitoneal injection of the 

 antiserum to the crystalline preparation. Three weeks after 

 the first injection of protein or human serum in the series of 

 actively sensitized animals, these guinea pigs were tested in two 

 ways for specific sensitivity. The reaction of the animal as a 

 whole was used when the intoxicating dose was given intravenously 

 or intraperitoneally, and the method of Schultz and Dale was 

 used with the uterine horns of the guinea pigs to provide graphic 

 records of the experiments. These reactions also demonstrated 

 (1) differences between the various Bence-Jones proteins, (2) a 

 mixture of human serum proteins and Bence-Jones proteins in 

 the preparations made in the attempt to salt-out Bence-Jones 

 protein from the urine, and (3) complete difference between the 

 crystalline Bence-Jones protein and human serum. (Demonstra- 

 tion of charts of precipitin and anaphylactic reactions.) 



112 (1694) 



On the influence of tissue enzymes on the bacteriophage principle. 



By ANN G. KUTTNER. 



[From the Department of Bacteriology, College of Physicians and 

 Surgeons, Columbia University.] 



I have previously reported before this Society the isolation of a 

 lytic principle active against typhoid and dysentery bacilli ob- 

 tained by the d'Herelle technique from the filtrate of a stool from 



