224 



Scientific Proceedings (115). 



extract. Small intestine extracted with 25 per cent, glycerine 

 has given similar results. Glycerine extracts of the large intes- 

 tine and of muscle tissue have so far given negative results. 



I prepared the liver extract according to the method used by 

 Turro in preparing tissue extracts. Turro has reported that 

 extracts of leucocytes, muscle tissue, kidneys, pancreas, thyroid, 

 etc., digest bacterial protein. He has worked particularly with 

 anthrax bacilli, but also with cholera and typhoid. He does not 

 in any of the papers that he has published up to this time, con- 

 nect his results with the phenomenon of d'Herelle, and does not 

 show that the dissolved bacteria can dissolve new cultures. He 

 states that no special ferment derived exclusively from poly- 

 nuclear leucocytes is necessary to digest bacteria, but that all 

 tissue cells probably contain such ferments. 



The liver extract was prepared in the following way: The 

 liver from a normal guinea pig was minced, shaken up with ace- 

 tone, dried in vacuo and pulverized. To approximately one 

 gram of liver powder, 20 c.c. of salt were added. To one tube, 

 40 drops of chloroform were then added and to the other, a small 

 amount of sodium fluoride. The tubes were placed in the in- 

 cubator for 14 hours. The tube with chloroform was sterile, the 

 tube to which the sodium fluoride was added, was contaminated, 

 both were centrifuged and the latter was filtered through a Berk- 

 feld. 



Both these liver extracts dissolve cultures of typhoid bacill 

 and the lytic principle can be transmitted in series from the dis- 

 solved culture. The 6th generation from the liver extract has 

 now been reached and the lytic action has increased both in the 

 degree of clearing and in the rapidity of the action. Cultures 

 are not sterilized completely and the two types of colonies, one, 

 the bearer of lytic principle, develop on plating. Control experi- 

 ments to determine whether it is the action of the glycerine on the 

 bacteria that produce the lysis have been negative. Similar con- 

 trol experiments with chloroform and sodium fluoride have up to 

 the present time not produced a transmittable lytic principle. 



These tissue extracts do not appear to be specific, but the 

 range of their action has not yet been determined. Both the 

 intestinal extract and the liver extract are active against several 



